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Chemical · Seminar 06 · Engineered enzymes that eat plastic

Enzymatic Plastic Recycling (PET Depolymerization)

Enzymatic recycling uses engineered enzymes to depolymerise PET plastic back into its pure monomers under mild conditions, enabling true closed-loop, infinite recycling.

enzymatic recyclingPETbiocatalysisdepolymerisationcircular economy

Mechanical recycling degrades plastic quality each cycle ('downcycling'). Enzymatic recycling offers a closed loop: engineered enzymes break PET (polyethylene terephthalate, the plastic of bottles and polyester) back into its building-block monomers, which can be repurified and re-polymerised into virgin-quality plastic — indefinitely.

Working principle

Specialised enzymes called PET hydrolases (e.g. engineered variants of leaf-branch compost cutinase, or IsPETase from a PET-digesting bacterium) catalyse hydrolysis of the ester bonds in the polymer chain. This cleaves PET into terephthalic acid (TPA) and ethylene glycol (EG). The reaction runs in water at mild temperatures (around the polymer's glass transition), consuming far less energy than chemical depolymerisation. Protein engineering boosts the enzyme's activity and thermostability.

PET waste1Pre-treat (amorphise)2Enzyme hydrolysis3TPA + ethylene glycol4Re-polymerise → new PET5Closed-loop enzymatic PET recycling
Figure 1. Enzymes cleave the ester bonds of PET into pure monomers that are repurified and polymerised back into bottle-grade plastic.
Table 1. Recycling routes for PET
RouteOutput qualityConditions
MechanicalDegrades each cycleMelt, simple
Chemical (glycolysis)MonomersHigh temp / catalyst
EnzymaticVirgin-grade monomersMild, aqueous, low energy
Why it mattersEnzymatic recycling enables a true circular economy for PET — infinite recycling without quality loss. Enzyme cost, reaction rate and tolerance to mixed/coloured waste are the scale-up hurdles.

Applications

  • Bottle-to-bottle and textile-to-textile closed-loop recycling
  • Processing coloured and mixed PET that mechanical recycling rejects
  • Recovering value from polyester textile waste

References & further reading

  1. Tournier et al., “An engineered PET depolymerase to break down and recycle plastic bottles,” Nature, 2020.
  2. Yoshida et al., “A bacterium that degrades and assimilates poly(ethylene terephthalate),” Science, 2016.
  3. Lu et al., “Machine learning-aided engineering of hydrolases for PET depolymerization,” Nature, 2022.